Isolation and Identification of Clostridium perfringens from Chickens and Their Drug Resistance
Objective:To investigate the isolation,identification and drug resistance of Clostridium perfringens of chicken origin in order to understand its biological characteristics and potential threats.Method:TSC AGAR,blood AGAR and ferrous sulfate milk culture medium were used to isolate and culture the characters and characteristics of Clostridium perfringens were analyzed systematically by morphological observation,biochemical characterization,PCR identification and drug resistance test.Result:The three isolates of Clostridium perfringens were identified as type A by PCR.The results of biochemical identification showed the diversity of carbon source metabolism.Toxin typing and 16S rRNA gene analysis confirmed the species identity and revealed their similarity at the molecular level.Resistance tests showed that these isolates were resistant to gentamicin,kanamycin,neomycin,polymycin B and cotrimoxazole,while highly sensitive to cefazolin,ceftriaxone,cefuroxime,ampicillin and piperacillin.Conclusion:This study comprehensively studied the molecular biological characteristics of Clostridium perfringens of chicken,emphasizing its diversity in carbon source metabolism and drug resistance,providing important basic data for the prevention and control of Clostridium perfringens infection,and providing scientific basis for further investigation of its pathogenic mechanism and rational use of antibiotics in the future.
Clostridium perfringens of chicken originisolation and identificationdrug resistance
万方数据
维普
