食品安全导刊2024，Issue(7) ：86-88,92.

固相萃取-高效液相色谱-串联质谱法同时测定海产品中微囊藻毒素和鱼腥藻毒素

Simultaneous Determination of Microcystins and Anatoxins in Marine Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction

吕晓静 鞠光秀 曲欣 汪勇 于红卫
食品安全导刊2024，Issue(7) ：86-88,92.
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固相萃取-高效液相色谱-串联质谱法同时测定海产品中微囊藻毒素和鱼腥藻毒素

Simultaneous Determination of Microcystins and Anatoxins in Marine Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction

吕晓静 1鞠光秀 1曲欣 1汪勇 1于红卫1
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作者信息

  • 1. 青岛市疾病预防控制中心/青岛市预防医学研究院,山东青岛 266033;岛津企业管理(中国)有限公司,北京 100020
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摘要

目的:建立固相萃取-高效液相色谱-串联质谱法同时测定海产品中 7 种微囊藻毒素和 2 种鱼腥藻毒素的方法.方法:样品经80%乙腈提取,HLB小柱净化后,采用MRM模式进行分析,外标法定量.结果:7 种微囊藻毒素和 2种鱼腥藻毒素在 0.5～50.0 μg·L-1 范围内线性关系良好,检出限为 0.3 μg·kg-1,回收率为 75.5%～98.8%,相对标准偏差在 1.5%～5.4%.结论:该方法重现性较好、灵敏度高、成本低,可以实现海产品中的鱼腥藻毒素和微囊藻毒素的同时检测.

Abstract

Objective:A method for simultaneous determination of 7 microcystins(MCs)and 2 Anatoxins(AnTXs)in marine products was achieved by solid phase extraction(SPE)-high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS).Method:The sample was extracted with 80%acetonitrile,purified by HLB small column,analyzed using MRM mode,and quantified using external standard method.Result:The linear ranges for 7 MCs and 2 AnTXs were 0.5～50.0 μg·L-1.The limits of detection were 0.3 μg·kg-1.The recoveries of the 7 MCs and 2 AnTXs spiked in blank marine products ranged from 75.5%to 98.8%with the relative deviations of 1.5%～5.4%.Conclusion:The method has the advantages of good reproducibility,high sensitivity and low cost,and can achieve simultaneous detection of fishy algae toxins and microcystins in seafood.

关键词

微囊藻毒素/鱼腥藻毒素/固相萃取(SPE)/高效液相色谱-串联质谱法(HPLC-MS/MS)

Key words

microcystin/anatoxin/solid phase extraction(SPE)/high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)

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出版年

2024
食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子：0.065
ISSN：1674-0270
参考文献量7
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