Simultaneous Determination of Microcystins and Anatoxins in Marine Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction
Objective:A method for simultaneous determination of 7 microcystins(MCs)and 2 Anatoxins(AnTXs)in marine products was achieved by solid phase extraction(SPE)-high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS).Method:The sample was extracted with 80%acetonitrile,purified by HLB small column,analyzed using MRM mode,and quantified using external standard method.Result:The linear ranges for 7 MCs and 2 AnTXs were 0.5～50.0 μg·L-1.The limits of detection were 0.3 μg·kg-1.The recoveries of the 7 MCs and 2 AnTXs spiked in blank marine products ranged from 75.5%to 98.8%with the relative deviations of 1.5%～5.4%.Conclusion:The method has the advantages of good reproducibility,high sensitivity and low cost,and can achieve simultaneous detection of fishy algae toxins and microcystins in seafood.
microcystinanatoxinsolid phase extraction(SPE)high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)
万方数据
维普
