This article uses ultra high performance liquid chromatography tandem mass spectrometry to analyze the residual levels of cephalosporins in pork.The sample was extracted by acetonitrile aqueous solution and phosphate buffer solution,purified by HLB solid-phase extraction column,and determined by ultra-high performance liquid chromatography mass spectrometry.The results showed that the correlation coefficient(R2)of the three cephalosporin drugs was greater than 0.996 within 0～200 ng·mL-1,indicating a good linear relationship;the detection limit is 0.001～0.365 μg·kg-1,with a quantification limit is 0.003～1.220 μg·kg-1.The spiked recovery rates of samples at different concentrations is 83.2%～95.2%,with a relative standard deviation is 1.9%～7.5%and a standard deviation is 0.078～2.817 μg·kg-1.
关键词
猪肉/头孢类药物/超高效液相色谱-串联质谱/固相萃取柱
Key words
pork/cephalosporins/ultra performance liquid chromatography tandem mass spectrometry/solid-phase extraction column
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