Isolation and Screening of Lactic Acid Bacteria Fermented with Short-Chain Fatty Acids from Wolfberry
Fifty strains of lactic acid bacteria were isolated from healthy human feces using MRS medium,and further screened on plates containing prebiotics to obtain 15 strains of lactic acid bacteria producing short-chain fatty acids.A comparison was made on the tolerance of the screened lactic acid bacteria to a simulated digestive environment,as well as their ability to utilize oligosaccharides for rapid screening and ferment Goji berries to produce short-chain fatty acids.One strain of lactic acid bacteria with good performance was selected,which exhibited good tolerance to simulated gastric and intestinal fluids,and strong ability to produce acetic acid,propionic acid,isobutyric acid,and butyric acid.Molecular biology identification revealed that this strain belonged to Limosilactobacillus fermentum.
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