基于产短链脂肪酸乳酸菌发酵枸杞分离筛选
Isolation and Screening of Lactic Acid Bacteria Fermented with Short-Chain Fatty Acids from Wolfberry
王燕燕 1黄远鹏 2张杰 2许雯 2张惠玲1
作者信息
- 1. 宁夏大学 食品科学与工程学院,宁夏银川 750021
- 2. 宁夏食品微生物应用技术与安全控制重点实验室,宁夏银川 750021
- 折叠
摘要
采用MRS培养基从健康人体粪便中分离得到 50 株乳酸菌,结合益生元筛选平板,进一步筛选获得 15 株产短链脂肪酸乳酸菌菌株.比较筛得的乳酸菌对模拟消化道环境的耐受性,以及在利用低聚糖快筛、发酵枸杞产短链脂肪酸等方面的能力差异,从中筛选得到一株性能良好的乳酸菌,其能较好地耐受模拟胃肠液,并具有较强的产乙酸、丙酸、异丁酸、正丁酸能力.通过分子生物学鉴定,该菌株为发酵乳酸杆菌Limosilactobacillus fermentum.
Abstract
Fifty strains of lactic acid bacteria were isolated from healthy human feces using MRS medium,and further screened on plates containing prebiotics to obtain 15 strains of lactic acid bacteria producing short-chain fatty acids.A comparison was made on the tolerance of the screened lactic acid bacteria to a simulated digestive environment,as well as their ability to utilize oligosaccharides for rapid screening and ferment Goji berries to produce short-chain fatty acids.One strain of lactic acid bacteria with good performance was selected,which exhibited good tolerance to simulated gastric and intestinal fluids,and strong ability to produce acetic acid,propionic acid,isobutyric acid,and butyric acid.Molecular biology identification revealed that this strain belonged to Limosilactobacillus fermentum.
关键词
短链脂肪酸/乳酸菌/枸杞/分离筛选Key words
train screening/lactic acid bacteria/Goji berries/Limosilactobacillus fermentum引用本文复制引用
基金项目
宁夏回族自治区重点研发计划项目(2022BBF02006)
出版年
2024
NETL
NSTL
万方数据