正交实验法优化杜仲叶发酵工艺
Optimization of Fermentation Process of Eucommia ulmoides Leaves by Orthogonal Experiment
张民科 1骆贤亮 2田梦 1冯凤琴 2张希1
作者信息
- 1. 云南中医药大学,云南昆明 650500
- 2. 浙江大学,浙江杭州 310058
- 折叠
摘要
以杜仲叶为原料,采用酵母菌进行发酵,利用单因素实验、正交实验结合隶属度综合评分法优化杜仲叶发酵工艺.结果表明,杜仲叶的最佳发酵工艺为料液比1∶11、发酵时间42 h、接种量5%.此条件下,杜仲叶发酵液芦丁含量为 0.33 mg·g-1、桃叶珊瑚苷含量为 0.37 mg·g-1、DPPH·清除率为 80.67%、·OH清除率为 5.21%.
Abstract
Using Eucommia ulmoides leaves as raw materials,yeast was used to ferment Eucommia ulmoides leaves.The fermentation process of Eucommia ulmoides leaves was optimized by single factor experiment,orthogonal experiment and comprehensive membership score method.The results showed that the optimum fermentation conditions of Eucommia ulmoides leaves were as follows:the ratio of material to liquid at 1∶11,fermentation time for 42 hours and inoculation amount of 5%.Under these conditions,the content of rutin in the fermentation broth of Eucommia ulmoides leaves was 0.33 mg·g-1,the content of aucubin was 0.37 mg·g-1,the clearance rate of DPPH·was 80.67%,and the clearance rate of·OH was 5.21%.
关键词
杜仲叶/真菌发酵/工艺优化/抗氧化Key words
Eucommia ulmoides leaves/fungal fermentation/process optimization/antioxidation引用本文复制引用
出版年
2024
NETL
NSTL
万方数据