Optimization of Fermentation Process of Eucommia ulmoides Leaves by Orthogonal Experiment
Using Eucommia ulmoides leaves as raw materials,yeast was used to ferment Eucommia ulmoides leaves.The fermentation process of Eucommia ulmoides leaves was optimized by single factor experiment,orthogonal experiment and comprehensive membership score method.The results showed that the optimum fermentation conditions of Eucommia ulmoides leaves were as follows:the ratio of material to liquid at 1∶11,fermentation time for 42 hours and inoculation amount of 5%.Under these conditions,the content of rutin in the fermentation broth of Eucommia ulmoides leaves was 0.33 mg·g-1,the content of aucubin was 0.37 mg·g-1,the clearance rate of DPPH·was 80.67%,and the clearance rate of·OH was 5.21%.
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