Objective:To establish a rapid analytical method for the determination of 12 common additives in food by high performance liquid chromatography.Method:After solid-phase extraction pretreatment,the sample was passed through a C18 chromatographic column(4.6 mm×250 mm,5 µm),with methanol and ammonium formate acetate as mobile phases for gradient elution,DAD detector detection,and external standard method for quantitative analysis.Result:The 12 additives had good linear correlation in the concentration range of 0.5～50.0 µg·mL-1,and the correlation coefficients were all higher than 0.999.The method detection limit was 0.008 0～0.095 2 mg·kg-1,and the recovery rate was 85.07%～104.18%,and the RSD was 0.6%～4.2%.Conclusion:This method is simple to operate,has high sensitivity,high resolution and fast analysis speed,and can complete the rapid detection of 12 additives in food within 16 minutes.
关键词
高效液相色谱法/防腐剂/甜味剂/着色剂
Key words
high performance liquid chromatography/preservative/sweetener/colorant
维普
万方数据