Rapid Determination of Ginsenoside Rh2 in Health Products by SPE-UPLC
Objective:To establish a rapid and accurate method for the determination of rare ginsenoside Rh2 in health food products based on solid-phase extraction coupled with ultra-high liquid chromatography(UPLC),and to provide a reference for the quality evaluation of commercially available health food products containing ginsenoside Rh2.Method:The samples were pretreated and extracted by methanol ultrasonication,purified by a neutral alumina SPE column,and screened for suitable eluents for the separation and purification on a HLB-SPE column,using a WatersT3(100 mm×2.1 mm,1.8 μm)chromatographic column with acetonitrile-water as the mobile phase in a gradient elution,and scanned by a PDA at full wavelength at a flow rate of 0.3 mL·min-1.Result:The concentration of ginsenoside Rh2 measured by SPE-UPLC showed a good linear relationship with its peak area in the range of 9.7～99.8 μg·mL-1(r=0.999),and the average recovery was 99.20%.Conclusion:The method is accurate,simple,rapid and effective in removing the interference of excipients,with high stability,and can be used for the quality control of health food containing ginsenoside Rh2 active ingredient.
万方数据
维普
