Objective:To establish a rapid and accurate method for the determination of rare ginsenoside Rh2 in health food products based on solid-phase extraction coupled with ultra-high liquid chromatography(UPLC),and to provide a reference for the quality evaluation of commercially available health food products containing ginsenoside Rh2.Method:The samples were pretreated and extracted by methanol ultrasonication,purified by a neutral alumina SPE column,and screened for suitable eluents for the separation and purification on a HLB-SPE column,using a WatersT3(100 mm×2.1 mm,1.8 μm)chromatographic column with acetonitrile-water as the mobile phase in a gradient elution,and scanned by a PDA at full wavelength at a flow rate of 0.3 mL·min-1.Result:The concentration of ginsenoside Rh2 measured by SPE-UPLC showed a good linear relationship with its peak area in the range of 9.7～99.8 μg·mL-1(r=0.999),and the average recovery was 99.20%.Conclusion:The method is accurate,simple,rapid and effective in removing the interference of excipients,with high stability,and can be used for the quality control of health food containing ginsenoside Rh2 active ingredient.
维普
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