Simultaneous Determination of 7 Illegal Additives in Flour by High Performance Liquid Chromatography-Tandem Mass Spectrometry
Objective:To establish a high performance liquid chromatography-tandem mass spectrometry method for the determination of 7 illegal additives in flour.Method:The samples were extracted by acetonitrile ∶ water(1∶1,V∶V),concentrated by ultrasound,centrifugation and nitrogen blowing,separated by Agilent Eclipse Plus C18 RRHD column,and then eluted with 0.1%formic acid-5 mmol·L-1 ammonium formate water and methanol as mobile phase,and analyzed by multi-reaction monitoring mode.Result:There was a good linear relationship(R2＞0.990)between 0.10～8.00 ng·mL-1 for the 7 target compounds.The limit of detection was 0.03～0.15 mg·kg-1,and the limit of quantification was 0.10～0.45 mg·kg-1.The recoveries were 88.6%～104.3%,and the relative standard deviations were 2.1%～5.8%(n=6).Conclusion:The method is simple,sensitive and accurate,and can satisfy the simultaneous detection of 7 illegal additives in wheat flour.
high performance liquid chromatography tandem mass spectrometrywheat flourillegal additivesthiobenzimidazolebenzohydroxamic acid
万方数据
维普
