Determination of 4 Synthetic Colorants in Sugar by HPLC-MS/MS
Objective:To establish an analytical method for the determination of four colorants in sugar using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Method:The sample was ultrasonically extracted with 80% methanol,purified with a PWA-2 solid phase extraction column,and then separated on a VP-ODS C18 column(2.0 mm×150 mm,5.0 μm)with a gradient elution using ammonium acetate and methanol as the mobile phases,and detected by HPLC-MS/MS.Result:The four compounds had good linearity within the investigation range,and the correlation coefficients were all greater than 0.999;the standard recovery rate was 85.36%～102.30%,and the RSD was 0.42%～2.72%;the detection limit was 2～5 μg·kg-1.Conclusion:This method has the characteristics of high sensitivity,resolution and fast analysis speed,and is suitable for rapid screening and quantitative analysis of synthetic colorants in sugar.
sugarhigh performance liquid chromatography-tandem mass spectrometrysynthetic colorantrapid determination
万方数据
维普
