Determination of Oleanolic Acid and Ursolic Acid in Papaya in Different Origin
Objective:To determine the content of oleanolic acid and ursolic acid in papaya from different regions.Method:Using papaya from Baoshan and Dali regions in Yunnan province as experimental materials,the extraction method was optimized through orthogonal experiments.High performance liquid chromatography was used to determine the content of oleanolic acid and ursolic acid in papaya.Result:The best extraction effect was achieved when the extraction reagent was methanol,soaking time was 1 h,and ultrasound time was 30 min.Conclusion:The main chemical components of papaya from different regions show differences in the content of oleanolic acid and ursolic acid,providing a theoretical basis for the determination of oleanolic acid and ursolic acid content in papaya.