Determination of Caffeine,Total Amount of Tea Polyphenols and Their Five Main Components in Tea Beverages by Using a One Test Multiple Evaluation Method
Objective:To establish a method for the determination of caffeine,total amount of tea polyphenols,catechin,gallic acid,epicatechin,epicatechin gallate and epicatechin in tea beverages.Method:HPLC was used to calculate the total amount of tea polyphenols and the relative correction factors of other 5 polyphenols,and to investigate the stability of the relative correction factors.The results of 7 indexes in tea beverage were compared between external standard method and one test and multiple evaluation method.Result:The relative correction factors of the 6 tested substances were catechin 0.34,gallic acid 1.41,epicatechin 0.86,epicatechin gallate 0.80,epicatechin 1.53,and total tea polyphenols 133.91,respectively.RSD of relative correction factors in different samples ranged from 2.95% to 8.78%,all of which were less than 10%,indicating good stability.There was no significant difference in the contents of 7 indexes in tea beverages obtained by one test and multiple evaluation method and external standard method.Conclusion:The established method is accurate and reliable,and can provide reference for the quality control of tea beverage.
tea beveragechromatographyone test with multiple evaluationstea polyphenolscaffeine
万方数据
维普
