首页|测试片法检测食品中大肠菌群的验证评价

测试片法检测食品中大肠菌群的验证评价

Validation of Coliform in Food by Test Tablets Method

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为探究大肠菌群测试片法在食品检测中的可行性,依据国际标准ISO 16140-2:2016 中的性能指标验证要求,对MicroFast®大肠菌群测试片法进行包容性、排他性,以及与国家标准《食品安全国家标准 食品微生物学检验 大肠菌群计数》(GB 4789.3-2016)中方法的一致性验证,采用t-检验和Bland-Altman分析方法进行一致性分析评价.16 株目标菌在测试片上形成典型菌落,24 株非目标菌中的 23 株均未在测试片上形成菌落,阿巴特图巴沙门氏菌ATCC 35640 在测试片上形成非典型菌落.测试片法和GB 4789.3-2016中方法的检测结果呈正相关(R2>0.976);95%置信区间的样品比例为 1∶25,满足不得高于 1∶20 的要求;所有食品类型的β-期望容忍区间(β-ETI)均在±0.5 对数单位范围内,测试片法和GB 4789.3-2016方法具有很好的一致性.MicroFast®大肠菌群测试片法对纯菌株的包容性和排他性良好,在一致性方面符合ISO 16140-2:2016对方法等效性的要求,可作为替代方法,用于检测食品中的大肠菌群.
This study was aimed to evaluate the feasibility of coliform detection in food by coliform test tablets.Based on performance indicator verification requirements of international standard ISO16140-2:2016,MicroFast® coliform test tablets method was used for evaluation,including inclusivity,exclusivity,and consistency with GB 4789.3-2016 method,and t-test and Bland-Altman analysis were used for consistency analysis and evaluation.The 16 target strains all grew typical colonies on the test tablets,and 23 of the 24 non-target strains had no colonies on the test tablets.Salmonella Abatetuba ATCC 35640 formed atypical colonies on the test tablets.There was a positive correlation between the test tablets method and the national standard method(R2>0.976).The proportion of samples exceeding the 95% confidence interval of the total average value of the difference was 1∶25,which met the ratio requirement of no more than 1 ∶ 20.The β-expected tolerance interval(β-ETI)for all food types was±0.5log10 units,the two methods were in good agreement.MicroFast® coliform count plate had good performance in terms of inclusivity and exclusivity to pure strains,met the requirements of ISO16140-2:2016 for method equivalence in terms of consistency.It can be used as an alternative method for testing coliforms in food.

test tablets methodcoliformsplate count method

陶文靖、董彬、焦凤莲、周琦、曲连海、王琦

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北京美正生物科技有限公司,北京 102200

北京光明健能乳业有限公司,北京 101300

通标标准技术服务(青岛)有限公司,山东青岛 266071

测试片法 大肠菌群 平板计数法

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
年,卷(期):2024.(10)
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