Validation of Coliform in Food by Test Tablets Method
This study was aimed to evaluate the feasibility of coliform detection in food by coliform test tablets.Based on performance indicator verification requirements of international standard ISO16140-2:2016,MicroFast® coliform test tablets method was used for evaluation,including inclusivity,exclusivity,and consistency with GB 4789.3-2016 method,and t-test and Bland-Altman analysis were used for consistency analysis and evaluation.The 16 target strains all grew typical colonies on the test tablets,and 23 of the 24 non-target strains had no colonies on the test tablets.Salmonella Abatetuba ATCC 35640 formed atypical colonies on the test tablets.There was a positive correlation between the test tablets method and the national standard method(R2＞0.976).The proportion of samples exceeding the 95% confidence interval of the total average value of the difference was 1∶25,which met the ratio requirement of no more than 1 ∶ 20.The β-expected tolerance interval(β-ETI)for all food types was±0.5log10 units,the two methods were in good agreement.MicroFast® coliform count plate had good performance in terms of inclusivity and exclusivity to pure strains,met the requirements of ISO16140-2:2016 for method equivalence in terms of consistency.It can be used as an alternative method for testing coliforms in food.
万方数据
维普
