Quantitative Determination of Aflatoxins B1 in Edible Oil by High Performance Liquid Chromatography
Objective:In order to establish the determination method of aflatoxins B1 in edible oil by high performance liquid chromatography.Method:The samples were extracted with methanol water,purified by aflatoxins B1 immunoaffinity column,eluted with methanol,and determined by post-column iodine derivatization.Result:The linear regression equation was Y=27 217.3X,the correlation coefficient was R2=0.999 6.The recovery rate of method was from 96.8%to 101.2%,the RSD of reproducibility was 0.6%,the Z value of quality control samples was 0.5,and the detection limit was 0.029 µg·kg-1,the limit of quantification was 0.098 µg·kg-1.Conclusion:The method has good linearity,good recovery,quality control sample,detection limit,quantitative limit are in line with the requirements,with simple,fast,efficient and save reagent consumables characteristics.
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