Study on Purification Process of Total Flavonoids from Paeonia rockii Seed Meal
D101,AB-8 and NKA-9 macroporous resins were selected for static adsorption and resolution performance tests,and AB-8 macroporous resin was screened for dynamic tests to obtain the optimal purification process conditions for total flavonoids in Paeonia rockii seed meal.The results show that the AB-8 macroporous resin was used for adsorption under the conditions of sample solution concentration of 5 mg·mL-1,pH value of 5,flow rate of 3 BV·h-1,and sample solution volume of 5 BV,and when the eluent is 70%ethanol,the eluent flow rate is 1.5 BV·h-1,and the eluent volume is 7 BV,the total flavonoid content of purified Paeonia rockii seed meal increases from 8.54%to 32.36%.This process has high feasibility and can provide an effective theoretical basis for the purification of total flavonoids in Paeonia rockii seed meal.
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