食品安全导刊2024,Issue(12) :136-139.

紫斑牡丹籽粕总黄酮纯化工艺研究

Study on Purification Process of Total Flavonoids from Paeonia rockii Seed Meal

陶波 杨文瑾
食品安全导刊2024,Issue(12) :136-139.

紫斑牡丹籽粕总黄酮纯化工艺研究

Study on Purification Process of Total Flavonoids from Paeonia rockii Seed Meal

陶波 1杨文瑾1
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作者信息

  • 1. 临夏现代职业学院 生物工程系,甘肃临夏 731100
  • 折叠

摘要

选用D101、AB-8、NKA-9 型大孔树脂进行静态吸附及解析性能试验,筛选出AB-8 型大孔树脂进行动态试验研究,获得了紫斑牡丹籽粕中总黄酮的最佳纯化工艺条件.结果显示,利用AB-8 型大孔树脂在上样液浓度为 5 mg·mL-1、pH值为 5、流速为3 BV·h-1、上样液体积为5 BV条件下进行吸附,在洗脱液为 70%乙醇、洗脱液流速为 1.5 BV·h-1、洗脱液体积为 7 BV条件下洗脱,纯化后紫斑牡丹籽粕总黄酮含量由 8.54%提高到32.36%,该工艺可行性较高,可为紫斑牡丹籽粕中总黄酮纯化提供有效理论依据.

Abstract

D101,AB-8 and NKA-9 macroporous resins were selected for static adsorption and resolution performance tests,and AB-8 macroporous resin was screened for dynamic tests to obtain the optimal purification process conditions for total flavonoids in Paeonia rockii seed meal.The results show that the AB-8 macroporous resin was used for adsorption under the conditions of sample solution concentration of 5 mg·mL-1,pH value of 5,flow rate of 3 BV·h-1,and sample solution volume of 5 BV,and when the eluent is 70%ethanol,the eluent flow rate is 1.5 BV·h-1,and the eluent volume is 7 BV,the total flavonoid content of purified Paeonia rockii seed meal increases from 8.54%to 32.36%.This process has high feasibility and can provide an effective theoretical basis for the purification of total flavonoids in Paeonia rockii seed meal.

关键词

紫斑牡丹籽粕/总黄酮/大孔树脂/纯化

Key words

Paeonia rockii seed meal/total flavonoids/macroporous resin/purification

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基金项目

2021年甘肃省高等学校创新基金项目(2021B-568)

出版年

2024
食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
参考文献量11
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