固相萃取结合液质联用法快速筛查和分析生活饮用水中的56种抗生素
Rapid Screening and Determination of 56 Antibiotics in Drinking Water by Solid-Phase Extraction Combined with UPLC-MS/MS
陈琨 1钟格梅 1韦日荣 1黄江平 1雷宁生 1廖艳华1
作者信息
- 1. 广西壮族自治区疾病预防控制中心,广西南宁 530028
- 折叠
摘要
目的:建立生活饮用中56种抗生素的固相萃取-液相色谱-串联质谱分析方法.方法:将生活饮用水通过大容量采样管连接到固相萃取柱上净化,用C18 柱梯度洗脱分离目标物,以乙腈和0.1%甲酸水为流动相,经电喷雾离子源电离,多反应监测模式串联质谱检测.结果:56种抗生素的最低检测浓度为0.1～2.0 µg·L-1,在其线性范围内的相关系数均大于 0.995,3 个浓度水平的加标回收率为 63.3%～113.0%,相对标准偏差在3.1%～8.3%.结论:该方法前处理简单、快速,适用于生活饮用水中多种抗生素的快速筛查和定量检测.
Abstract
Objective:To develop a method for determination of 56 antibiotics in drinking water by solid-phase extraction and UPLC-MS/MS.Method:Drinking water is connected to the solid phase extraction column for purification through a large-capacity sampling tube,target object were separated on C18 with gradient elution using acetonitrile-0.10%formic acid as mobile phase and detected by MS/MS using positive electrospray ionization under the multiple reaction monitoring.Result:The minimum detection of 56 antibiotics was 0.1～2.0 µg·L-1.The correlation coefficients in its linear range are over 0.995.The recovery rates of the 3 concentration levels were 63.3%～113.0%with the relative standard deviations of 3.1%～8.3%.Conclusion:The method has simple and rapid pretreatment,and is suitable for rapid screening and quantitative detection of multiple antibiotics in drinking water.
关键词
超高效液相色谱-串联质谱/固相萃取/生活饮用水/大容量采样管Key words
ultra performance liquid chromatography-tandem mass spectrometry/solid-phase extraction/drinking water/large-capacity sampling tube引用本文复制引用
基金项目
广西壮族自治区医药卫生计划(Z2015448)
出版年
2024
NETL
NSTL
万方数据