Study on the Resistance of Escherichia coli from Food Sources in Agricultural Markets and Its ESBL Resistance Genes
Objective:To investigate the contamination of ESBL in Escherichia coli sold in livestock and poultry meat,eggs and vegetables in Baoshan city,Yunnan province,and to study the types of extended-spectrum β-lactamase(ESBL)resistance genes of Escherichia coli isolated from the samples.Method:Food samples were collected in Baoshan farmers'market,and Escherichia coli was isolated and cultured for identification and drug susceptibility test,and ESBL gene was amplified by PCR,sequenced and analyzed for homology comparison.Result:A total of 75 strains of Escherichia coli were isolated from 245 food specimens,including 25 strains of ESBL E.coli.Phylogenetic analysis of the sequencing results of ESBL resistance genes showed that the drug resistance genes were CTX-M type,and there were 9 strains in the CTX-M-15 group,accounting for 36%.There were 16 strains in the CTX-M-14 group,and the detection rate was 64%.Conclusion:Meat and vegetables carry ESBL Escherichia coli,and multidrug-resistant bacteria and their resistance genes may be transmitted in the community through food.
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