无花果可溶性糖含量测定
Method for Determination of Soluble Sugar Content in Fig
苏云婷 1潘世会2
作者信息
- 1. 威海职业学院,山东威海 264200
- 2. 山东大学,山东威海 264209
- 折叠
摘要
为了建立一个可靠且高效的测定方法,专门用于测量不同成熟度无花果中的可溶性糖含量,深入探索并精确改进蒽酮-硫酸比色法.根据研究目的,针对性优化了显色时间、蔗糖标准液浓度与冷却比色时间,建立了可用于无花果果实可溶性糖含量测定的蒽酮-硫酸比色法.实验结果显示,最佳显色时间为10 min,最佳蔗糖标准溶液浓度为 100 μg·mL-1,最佳冷却比色时间为 20 min.回归方程y=0.005x+0.153 4(R2=0.998 2).通过检测不同成熟度的无花果,发现糖度变化趋势与可溶性糖含量变化趋势一致,可溶性糖含量可作为无花果产品质量控制指标.
Abstract
In order to establish a reliable and efficient assay specifically for measuring the soluble sugar content in figs of different ripenesses,the anthone-sulfate colorimetric method was explored in depth and precisely improved.According to the purpose of the study,the color development time,the concentration of sucrose standard solution and the cooling colorimetric time were optimized,and an anthone-sulfate colorimetric method was established for the determination of soluble sugar content in fig fruit.The experimental results showed that the optimal color development time was 10 min,the optimal concentration of sucrose standard solution was 100 μg·mL-1,and the optimal cooling colorimetric time was 20 min.The regression equation y=0.005x+0.153 4(R2=0.998 2).By detecting figs with different ripeness,it was found that the change trend of sugar content was consistent with the change trend of soluble sugar content,and the soluble sugar content could be used as a quality control index for fig products.
关键词
无花果/可溶性糖/蒽酮-硫酸比色法Key words
fig/soluble sugar/anthrone-sulfuric acid colorimetry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据