Research on the Isolation and Purification Process of Novel Food Additive PQQ
In order to optimize the adsorption and elution steps of macroporous resin in the separation and purification process of the new food additive pyrroloquinoline quinone(PQQ)fermentation supernant and improve the elution yield,XAD-1600 macroporous resin was used to adsorb and eluate PQQ in the upper column liquid.The elution yield was calculated by optimizing the pH value of the upper column liquid,the concentration of sodium chloride in the eluent,the elution temperature and the elution flow rate.The results showed that under the conditions of pH value of PQQ upper column solution was 3.5,phosphate buffer was selected as elution agent,sodium chloride content in phosphate buffer was 1.4%,elution flow rate was 2 BV·h-1,and elution temperature was 20℃,the elution yield of PQQ was the highest(96.7%).
new food additivespyrroloquinoline quinoneseparation and purificationmacroporous resinelution yield
NETL
NSTL
万方数据
