Determination of 18 Free Amino Acids in Instant Hydrolyzed Whey Protein Powder by Pre-Column Derivatization Coupled with High Performance Liquid Chromatography
The simultaneous determination of 18 kinds of free amino acids in instant hydrolyzed whey protein powder by pre-column derivatization and high performance liquid chromatography was studied.The results showed that the amino acid chromatographic peaks were well separated,the correlation coefficients of 18 amino acid regression equations were all above 0.998,the recoveries were 92.6%～107.4%,and the relative standard deviations were less than 5.0%.This method can be used to determine the content of commercially available instant hydrolyzed whey protein,and the operation is simpler than GB 5009.124-2016,and the determination results are good.
pre-column derivatization6-aminoquinolyl-N-hydroxysuccinimidyl-carbamatefree amino acidsinstant hydrolyzed whey protein
NETL
NSTL
万方数据
