摘要
目的:建立一种液相色谱法测定食品中合成着色剂的方法.方法:用乙醇氨水溶液提取食品中的合成着色剂,经固相萃取柱净化后,用液相色谱仪(配二极管阵列检测器)测定,外标法定量.结果:11 种合成着色剂在 0.2～10.0 μg·mL-1 线性关系良好,线性相关系数为 0.9994～1.0000,加标回收率为94.2%～109.6%,相对标准偏差在0.55%～5.52%.柠檬黄、喹啉黄、日落黄、胭脂红、新红和赤藓红的检出限均为0.5 mg·kg-1,定量限均为1.5 mg·kg-1;苋菜红、诱惑红、亮蓝、酸性红和靛蓝的检出限均为0.3 mg·kg-1,定量限均为 1.0 mg·kg-1.结论:该方法的重复性好、灵敏度高、实用性强,可以同时测定食品中多种合成着色剂的含量.
Abstract
Objective:To establish a method for the determination of synthetic colorants in food by liquid chromatography.Method:The synthetic colorants in food were extracted with ethanol ammonia solution,purified by solid phase extraction column,determined by liquid chromatography(with diode array detector),and quantified by external standard method.Result:The linear range of 11 synthetic colorants was 0.2～10.0 μg·mL-1,the linear correlation coefficient was 0.9994～1.0000,the recovery rate was 94.2%～109.6%,and the relative standard deviation was 0.55%～5.52%.The detection limits of tartrazine,quinoline yellow,sunset yellow,carmine,new red and erythrosine were 0.5 mg·kg-1,and the limits of quantification were 1.5 mg·kg-1.The limits of detection of amaranth,allura red,brilliant blue,acid red and indigo were 0.3 mg·kg-1,and the limits of quantitation were 1.0 mg·kg-1.Conclusion:The method has good repeatability,high sensitivity and strong practicability,and can simultaneously determine the content of various synthetic colorants in food.
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