为评价梨汁果味凉茶的抗氧化能力,测定了梨汁果味凉茶对DPPH自由基、羟自由基、超氧阴离子等的清除率,梨汁果味凉茶的总酚含量与铁离子还原/抗氧化能力法(Ferric Ion Reducing Antioxidant Power,FRAP).结果表明,清除DPPH自由基、羟自由基、超氧阴离子的IC50 值分别为 17.06%、79.08%、64.44%;总酚含量为(62.676±0.418)µg GAE·mL-1.随着梨汁果味凉茶浓度的增大,梨汁果味凉茶中DPPH自由基清除率逐渐下降,但羟自由基清除率、超氧阴离子清除率逐渐提高以及FRAP值逐渐增大.DPPH自由基清除率、羟自由基清除率、超氧阴离子清除率以及FRAP法两两之间均具有显著的正相关性.
The Research on the Antioxidant Activity of Pear-Flavoured Herbal Tea
To evaluate the antioxidant activity of pear-flavored herbal tea,the research measured scavenging rate of DPPH radicals,hydroxyl radicals and superoxide anions,the total phenol content and FRAP.The result showed that the IC50 value of DPPH radicals clearance rate was 17.06%,the IC50 value of hydroxyl radicals clearance rate was 79.08%,the IC50 value of superoxide anions was 64.44%,and the total phenol content was(62.676±0.418)μg GAE·mL-1.As the concentration of pear juice fruit flavored herbal tea increases,the DPPH radical scavenging rate in pear juice fruit flavored herbal tea gradually decreases,but the hydroxyl radical scavenging rate,superoxide anion scavenging rate,and FRAP value gradually increase.There is a significant positive correlation between DPPH radical scavenging rate,hydroxyl radical scavenging rate,superoxide anion scavenging rate,and FRAP method.
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