测试片法在食品菌落总数检测中的应用可行性分析
Feasibility Analysis of the Application of Test Slice Method in the Detection of Total Number of Food Colonies
杨静1
作者信息
- 1. 陕西省产品质量监督检验研究院,陕西西安 710048
- 折叠
摘要
为评估测试片法在食品菌落总数检测中的应用可行性及与传统的平板计数法的一致性,选取大肠埃希氏菌、枯草芽孢杆菌和蜡样芽孢杆菌作为标准菌株,同时采集生肉、水产品、乳制品、面食和果蔬等自然污染样品,分别采用测试片法和平板计数法对上述样品进行菌落总数检测,并采用t检验分析两种方法测定结果的一致性.结果表明,两种方法对标准菌株的检测结果无显著差异(P＞0.05),对自然污染样品的检测也无显著差异(P＞0.05),其中现制面条菌落总数最多,牛肉菌落总数最少.这表明测试片法与平板计数法的检测结果具有很好的一致性.测试片法操作简便,为食品菌落总数快速检测提供了可行的技术手段,具有一定的应用前景.
Abstract
In order to evaluate the application feasibility of the test slice method in the detection of the total number of food colonies and the consistency with the traditional plate counting method,Escherichia coli,Bacillus subtilis and Bacillus cereus are selected as the standard strains,and natural contaminated samples such as raw meat,aquatic products,dairy products,pasta and fruits and vegetables are collected.The total number of colonies are detected by the test slice method and the plate counting method,and t-test is used to analyze the consistency of the two methods.The results show that there is no significant difference between the two methods in the detection of standard strains(P＞0.05),and there is no significant difference in the detection of natural contaminated samples(P＞0.05),in which the total number of fresh noodle colonies is the highest,and the total number of beef colonies is the least.This shows that the test results of the test slice method and the plate counting method are in good agreement.The test slice method is easy to operate and provides a feasible technical means for the rapid detection of the total number of food colonies,and has a certain application prospect.
关键词
测试片法/平板计数法/食品/菌落总数Key words
test slice method/plate counting method/food/total colony引用本文复制引用
出版年
2024
NETL
NSTL
万方数据