Study on a Recombinase Polymerase Amplification Assay for Rapid Detection of Pseudomonas aeruginosa
Based on the highly conserved exotoxin A gene in Pseudomonas aeruginosa,specific primers were designed to establish and optimize the amplification conditions of recombinase polymerase amplification(RPA),the sensitivity and anti-interference ability of the method were evaluated.The results showed that the RPA reaction system could achieve the amplification of the target gene within 20 minutes under the isothermal condition of 40℃,and the sensitivity of detecting Pseudomonas aeruginosa was 102 CFU·mL-1,and the anti-interference ability was consistent with GB 8538-2022.After culture optimization,the minimum detection limit of Pseudomonas aeruginosa could reach 10 CFU·mL-1,and the repeatability was good.The RPA amplification detection method of Pseudomonas aeruginosa established in this study has the advantages of simple operation,rapid response and strong anti-interference ability,which provides a new direction and reference for the rapid detection of Pseudomonas aeruginosa in food.
NETL
NSTL
万方数据
