一起由副溶血性弧菌引起的食物中毒事件的病原检测及溯源分析
Etiological Survey and Traceability Analysis of a Food Poisoning Incident Caused by Vibrio parahaemolyticus
张阳 1李东迅 1王维钧 1牛桓彩 1谷田 1舒高林1
作者信息
- 1. 北京市昌平区疾病预防控制中心,北京 102206
- 折叠
摘要
目的:对一起农村自办婚宴发生的食物中毒事件进行病原检测及溯源分析.方法:对采集的粪便、肛拭子、手涂抹样等样本进行病原学检测,对检出的副溶血性弧菌进行血清凝集、3 种毒力基因检测、MIC药敏试验和PFGE分型.结果:该起食物中毒事件共涉及 10 例病例,发病时间较集中,首末病例发病时间间隔 4.75 h,患者主要症状为腹痛、腹泻、恶心、呕吐等.采集的样本中共检出 5 株副溶血性弧菌,分为3种血清型,4种PFGE型,毒力基因均为tlh+tdh+trh-,对头孢唑啉全部耐药.结论:该起农村自办婚宴食物中毒事件由副溶血性弧菌感染引起.
Abstract
Objective:To conduct the laboratory test and traceability analysis on a food poisoning at a rural self hosted wedding banquet.Method:Pathogens were tested from stool samples,anal swabs and hand applied swabs collected.Serotyping,3 types of virulent gene detection,broth microdilution method and PFGE typing tests were performed on Vibrio parahaemolyticus strains.Result:There were 10 cases in this epidemic,the interval between the onset of the first and last cases is 4.75 h.Their clinical symptoms mainly included abdominal pain,diarrhea,nausea and vomiting.A total of 5 Vibrio parahaemolyticus strains were detected and identified as three serotypes,four PFGE patterns and the virulence genes profiles were tlh+tdh+trh-.All five strains were resistance to cefazolin.Conclusion:This foodborne disease event was caused by Vibrio parahaemolyticus at a rural self hosted wedding banquet.
关键词
副溶血性弧菌/血清型/脉冲场凝胶电泳/耐药性/毒力基因Key words
Vibrio parahaemolyticus/serotypes/pulsed field gel electrophoresis/drug resistance/virulence genes引用本文复制引用
出版年
2024
NETL
NSTL
万方数据