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黑龙江省沙门氏菌血清型鉴定及两种血清分型方法对比

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目的:分析沙门氏菌血清型分子鉴定试剂盒的分型效果,探寻一种新型高效的血清型鉴定方法.方法:采用沙门氏菌血清分子鉴定方法和经典血清凝集法分别对从黑龙江省食品监测和食源性疾病人群分离得到的 69 株沙门氏菌、37 株沙门氏菌标准菌株以及往年分离并保藏的 100 株不同血清型沙门氏菌进行分型鉴定,对比两种分型方法的结果一致性.结果:两种分型方法成功率均为 100%,结果的符合率为 99.5%.其中来自黑龙江省的疾病人群分离的 69 株沙门氏菌共有 10 个血清型.结论:沙门氏菌血清型分子鉴定试剂盒与经典分型方法的一致性较高,且操作简便、准确性高,有望得到广泛应用.
Identification of Salmonella Serotypes in Heilongjiang Province and Comparison of Two Serotyping Methods
Objective:To analyze the typing effect of Salmonella serotype molecular identification kit and to explore a new and efficient serotype identification method.Method:69 strains of Salmonella,37 standard strains of Salmonella and 100 strains of Salmonella serotypes isolated and preserved in previous years were identified by using Salmonella serum molecular identification method and classical serum agglutination method,respectively,and the results of the two classification methods were compared.Result:The success rate of both classification methods was 100%,and the coincidence rate of the results was 99.5%.Among them,69 strains of Salmonella isolated from the disease population in Heilongjiang province had a total of 10 serotypes.Conclusion:The Salmonella serotype molecular identification kit has high consistency with the classical typing method,and is easy to operate and highly accurate,which is expected to be widely used.

Salmonellaserotypingidentify

李鑫、杨红霞、仲伟麒、任启智、白晶、张宇亮、季丽男

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黑龙江省疾病预防控制中心,黑龙江哈尔滨 150030

哈尔滨市松北区疾病预防控制中心,黑龙江哈尔滨 150030

黑龙江省传染病防治院,黑龙江哈尔滨 150030

沙门氏菌 血清分型 鉴定

黑龙江省卫生健康委科技项目(2023)

20231212070191

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
年,卷(期):2024.(16)
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