One Stop QuEChERS Method Combined with UPLC-MS/MS for the Determination of 18 Species in Animal Food β-Receptor Agonist Residue
Objective:To Establish a one-stop QuEChERS automatic extraction and purification pre-treatment system,combined with ultra-high performance liquid chromatography tandem mass spectrometry to simultaneously determine the residues of 18 β-receptor agonists in animal food.Method:Add 0.2 mol·L-1 ammonium acetate buffer to the sample,and β-Glucosidase/aryl sulfatase enzymolysis was extracted and purified by the QuEChERS automatic sample processing system.WATERS pentafluorophenyl column chromatography column(50 mm×3.0 mm,2.5 µm),formic acid aqueous solution(0.1%)and formic acid acetonitrile solution(0.1%)were used as the liquid chromatography mobile phase for gradient elution,tandem mass spectrometry electrospray ESI+mode,multiple reaction monitoring mode for determination,and internal standard method for quantification.Result:The linear correlation coefficients of 18 types β-receptor agonists within the concentration range of 0.1～20.0 ng·mL-1 are all greater than 0.995 5,and the detection limit is between 0.025～0.100 μg·kg-1.The spiked recovery rates of three levels in negative samples are 76.5%～106.8%,and the relative standard deviation is 0.2%～3.8%(n=6).Conclusion:This method is safe,accurate,and efficient,and can be used for the rapid detection of 18 β-receptor agonist residues in animal food in large quantities,providing a basis for establishing a QuEChERS pre-treatment method.
QuEChERSultra high performance liquid chromatography tandem mass spectrometryanimal based foodsβ-receptor agonists
NETL
NSTL
万方数据
