顶空固相微萃取-气相色谱-氢火焰离子检测法测定食用油中苯并(a)芘含量研究
Study on the Determination of BaP Content in Edible Oils by Headspace Solid-Phase Microextraction-Gas Chromatography-Hydrogen Flame Ionization Detection Method
罗小珍 1李夸巧1
作者信息
- 1. 罗平县检验检测所,云南罗平 655800
- 折叠
摘要
目的:建立一种快速、灵敏、可靠的顶空固相微萃取-气相色谱-氢火焰离子法(Headspace Solid-Phase Microextraction-Gas Chromatography-Hydrogen Flame Ionization Detection,HS-SPME-GC-FID)检测食用油中苯并(a)芘含量的分析方法,并应用于市售食用油样品的测定.方法:采用HS-SPME技术萃取油样中的苯并(a)芘,结合GC-FID进行分离检测和定量分析.考察方法的线性范围、精密度、准确度、检出限和定量限等指标,并测定 5 种市售食用油样品中的苯并(a)芘含量.结果:苯并(a)芘在 0.01～10.00 μg·mL-1 时呈良好的线性关系,精密度符合要求.5 种食用油样品苯并(a)芘含量在 0.127～0.452 μg·kg-1,均低于限量标准.结论:建立的HS-SPME-GC-FID分析方法操作简便、快速灵敏,可满足食用油中苯并(a)芘日常检测和食品安全监管需求.
Abstract
Objective:To establish a rapid,sensitive,and reliable method for detecting benzo(a)pyrene content in edible oils using headspace solid phase microextraction-gas chromatography-hydrogen flame ion detection method(HS-SPME-GC-FID)and apply it to the determination of commercially available edible oil samples.Method:HS-SPME technique was employed to extract benzo(a)pyrene from oil samples,combined with GC-FID for separation,detection,and quantitative analysis.Analytical performance indicators such as linear range,precision,accuracy,limit of detection,and limit of quantification were evaluated,and the benzo(a)pyrene content in five commercially available edible oil samples was determined.Result:Benzo(a)pyrene showed a good linear relationship in the concentration range of 0.01～10.00 μg·mL-1,and the precision met the standard requirements.The benzo(a)pyrene content in the five edible oil samples ranged from 0.127 μg·kg-1 to 0.452 μg·kg-1,all below the limit standard.Conclusion:The established HS-SPME-GC-FID analysis method is simple to operate,rapid,and sensitive,and can meet the requirements for routine detection of benzo(a)pyrene in edible oils and food safety supervision.
关键词
食用油/苯并(a)芘/顶空固相微萃取/食品安全Key words
edible oil/benzo(a)pyrene/headspace solid phase microextraction/food safety引用本文复制引用
出版年
2024
NETL
NSTL
万方数据