Application of Liquid Chromatography-Tandem Mass Spectrometry in the Detection of β-Receptor Agonist Drug Residues in Meat Products
β-receptor agonist drugs still pose a risk of illegal use in animal husbandry,posing a potential threat to consumer health.We used liquid chromatography-tandem mass spectrometry(LC-MS/MS)to detect β-receptor agonist drugs in pork.After enzymatic digestion,purification and concentration,the samples were subjected to qualitative and quantitative analysis using LC-MS/MS in multiple reaction monitoring(MRM)mode.The optimized pretreatment conditions were methanol-water(70∶30,v/v)as the extraction solvent,enzymatic hydrolysis of β-glucuronidase water bath at 37℃for 12 h,and solid-phase extraction and purification.The optimized instrument conditions were gradient elution using a C18 column with 0.1%formic acid in water and acetonitrile as mobile phases.The four β-receptor agonists had a good linear relationship with the response value in the given concentration range,and the correlation coefficient was greater than 0.99,the intra-day and inter-day relative standard deviation was less than 10%,the recovery rate was 87.9%to 92.5%,and the matrix effect value was 90.4%to 109.7%.Methodological validation shows that the method meets the requirements for detecting β-receptor agonist drug residues in meat products.
liquid chromatography-tandem mass spectrometry technologyβ-receptor agonistresiduemeat products
NETL
NSTL
万方数据
