Study on the Preparation Conditions of Immobilized Pancreatic Lipase Based on Magnetic Nanoparticles
Pancreatic lipase(PL),as an efficient biocatalyst and key target for treating obesity,has been widely used in the food industry and pharmaceutical fields.However,the stability of free pancreatic lipase is poor,and its activity is difficult to maintain and reuse.In this study,magnetic nanoparticles(MNPs)were used as carriers,and a PL-functionalized MNPs(PL-MNPs)screening model was constructed by using the affinity between MNPs and PL.The synthesis conditions of PL-MNPs were optimized by single factor and response surface methodology based on the enzyme immobilized capacity and relative enzyme activity of PL.The optimum synthesis conditions were determined as follows:pH=6.53,enzyme concentration 25.28 mg·mL-1,immobilization temperature 30.69℃and immobilization time 3.00 h.Under these conditions,the theoretical enzyme immobilized capacity was 144.95 μg·mg-1 and the relative enzyme activity was 94.27%.
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