基于磁性纳米粒子的固定化胰脂肪酶制备条件的研究
Study on the Preparation Conditions of Immobilized Pancreatic Lipase Based on Magnetic Nanoparticles
刘翠翠 1陈雨实2
作者信息
- 1. 马鞍山市食品药品安全检查中心,安徽马鞍山 243000
- 2. 福州大学 化学学院,福建福州 350000
- 折叠
摘要
胰脂肪酶(Pancreatic Lipase,PL)作为一种高效的生物催化剂和治疗肥胖的关键靶点,已经在食品工业和医药领域广泛应用,但游离胰脂肪酶稳定性较差,活力不易保持且难以重复利用.本研究以磁性纳米粒子(Magnetic Nanoparticles,MNPs)为载体,利用MNPs与PL的亲和作用,构建了PL功能化的MNPs(PL-MNPs)筛选模型.以PL的酶固载量和相对酶活为考察指标对PL-MNPs的合成条件进行单因素和响应面优化,确定PL-MNPs的最佳合成条件为pH=6.53,酶浓度 25.28 mg·mL-1,固定化温度 30.69℃,固定化时间 3.00 h.在此条件下,理论酶固载量为144.95 μg·mg-1,相对酶活为94.27%.
Abstract
Pancreatic lipase(PL),as an efficient biocatalyst and key target for treating obesity,has been widely used in the food industry and pharmaceutical fields.However,the stability of free pancreatic lipase is poor,and its activity is difficult to maintain and reuse.In this study,magnetic nanoparticles(MNPs)were used as carriers,and a PL-functionalized MNPs(PL-MNPs)screening model was constructed by using the affinity between MNPs and PL.The synthesis conditions of PL-MNPs were optimized by single factor and response surface methodology based on the enzyme immobilized capacity and relative enzyme activity of PL.The optimum synthesis conditions were determined as follows:pH=6.53,enzyme concentration 25.28 mg·mL-1,immobilization temperature 30.69℃and immobilization time 3.00 h.Under these conditions,the theoretical enzyme immobilized capacity was 144.95 μg·mg-1 and the relative enzyme activity was 94.27%.
关键词
胰脂肪酶/磁性纳米粒子/固定化酶Key words
pancreatic lipase/magnetic nanoparticles/immobilized enzymes引用本文复制引用
出版年
2024
NETL
NSTL
万方数据