Research of Burkholderia gladioli in Food by Real-Time Fluorescent PCR Method
Objective:To establish a method for Burkholderia gladioli DNA detection.Method:Based on 16S～23S rRNA gene fragment sequence,TaqMan probes and primers were designed by Oligo7.The method of real-time fluorescent PCR detection was further verified for its specificity and sensitivity.Result:Detecting 15 standard strains by real-time fluorescent PCR detection method,only Burkholderia gladioli was positive.So it's confirmed that this method has good specificity.Through sensitivity experiments of real-time fluorescent PCR method,it was detected that the sensitivity of the bacterial solution was 5.8×102 CFU·mL-1 and that of DNA was 7.2×10-4 ng·μL-1.Conclusion:The established real-time fluorescent PCR method has strong specificity and sensitivity so that it can be applied to rapid detection of Burkholderia gladioli,demonstrating good application value.
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