Objective:To establish an high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of multiple growth regulators in fruits and vegetables.Method:After optimising the sample extraction and clean-up conditions to improve the sensitivity and accuracy of the detection of target compounds,acetonitrile combined with QuEChERS extraction was used for the sample pre-treatment and clean-up by a C18 solid phase extraction column.The mass spectrometer used an electrospray ion source to optimise the scanning range and collision energy of the parent and daughter ions.Result:The optimized extraction and purification conditions can improve the extraction rate of growth agents,with a calibration curve R2≥0.996.Conclusion:The method can effectively improve the detection sensitivity,is simple to operate,and is suitable for the analysis of growth regulator residues in fruits and vegetables.
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)fruits and vegetablesgrowth regulators
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