Determination of 13 Glucocorticoids in Fish by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
A method to determine 13 glucocorticoids in fish using QuEChERS combined with ultra-high performance liquid chromatography-tandem mass spectrometry was developed.By optimizing pre-processing methods and conducting methodological validation,the results indicated that the 13 glucocorticoids exhibited good linearity within the range of 1 ng·mL-1 to 100 ng·mL-1,with correlation coefficients all greater than 0.99,the limits of quantification ranged from 0.2 μg·kg-1 to 5.0 μg·kg-1,and the recovery rates were between 75.1%and 95.7%.This method has simple and fast pre-processing,high recovery rate and precision,and can be used for the detection of 13 glucocorticoids in fish meat.
QuEChERSultra-high performance liquid chromatography-tandem mass spectrometryglucocorticoidsfish
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