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酸汤中产酸酵母的筛选及性能测定

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产酸酵母作为酸汤发酵过程中的优势菌株,是影响酸汤产品品质的关键因素.本文通过形态学、生理生化和分子生物学,对从酸汤样品中分离出的产酸菌株进行鉴定,并测定分析其产酸、耐酸和耐胆盐能力.结果表明,RS-14 为盔状毕赤酵母(Pichia galeiformis),CS-16 和CS-19 为膜醭毕赤酵母(Pichia membranaefaciens),45、RS-13、RS-15、CS-21 和CS-22 为满洲毕赤酵母(Pichia manshurica).其中,菌株 45 的产酸能力最强,总酸含量达到 41.47 g·L-1,且 8 株酵母菌均能在pH值为 1.5 和牛胆盐浓度为 0.70%的条件下生长,具有较高的耐酸性能和耐牛胆盐性能.本研究通过对酸汤中产酸酵母的筛选及其性能测定,为开发酸汤纯种发酵菌株和提升酸汤发酵产品品质提供理论支撑.
Screening and Performance Determination of Acid-Producing Yeast in Sour Soup
Acid-producing yeast,as the dominant strain in the fermentation process of sour soup,is the key factor affecting the quality of sour soup products.In this paper,the acid-producing strains isolated from sour soup samples were identified by morphology,physiology,biochemistry and molecular biology,and their acid production,acid resistance and bile salt resistance were determined and analyzed.The results showed that RS-14 was Pichia galeiformis,CS-16 and CS-19 were Pichia membranacefaciens,and 45、RS-13、RS-15、CS-21 and CS-22 were Pichia manshurica.Among them,strain 45 has the strongest acid production capacity,with a total acid content of 41.47 g·L-1,and all 8 strains of yeast can grow under the conditions of pH 1.5 and bile salt concentration of 0.70%,with high acid and bile salt resistance.In this study,the screening and performance determination of acid-producing yeast in sour soup provided theoretical support for the development of pure fermentation strains of sour soup and the improvement of the quality of sour soup fermentation products.

yeastsour souporganic acidbile salt tolerance

潘宇、杨第芹、朱霞、叶雨、杨熟英

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贵州省生物技术研究开发基地有限公司,贵州贵阳 550025

酵母菌 酸汤 有机酸 耐胆盐

2024

食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子:0.065
ISSN:1674-0270
年,卷(期):2024.(21)