Determination of Four Nitrofuran Metabolites in Animal Derived Food by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
In this research,an ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was developed for the simultaneous determination of 3-amino-2-oxazolidinone(AOZ),5-morpholinomethyl-3-amino-2-oxazolidinone(AMOZ),semicarbazide(SEM)and 1-aminohydontion(AHD)residues in food from animal origin.These samples were treated with 0.2 mol·L-1 hydrochloric acid water as an extract and 2-nitrobenzaldehyde as derivative.Samples were shaken in a water bath at 37℃for 4 h,and the pH was adjusted to 7.4±0.2,and purified by HLB solid phase extraction column,then dried by nitrogen,used 0.1%formic acid water to redissolve(included 50%methanol),0.1%formic acid water are mixed with 10 mmol·L-1 ammonium acetate and methanol as mobile phase to gradient.C18 column to separation,scanning electrospray positive in ion mode and multi-reaction monitoring mode,quantification by internal standards.The results showed that the four nitrofuran drugs had good linearity,and correlation coefficients(R2)were all greater than 0.999.The detection limits of AOZ,AMOZ,SEM and AHD in animal food were all 0.2 μg·kg-1,and quantitation limits were 0.4 μg·kg-1.At low,medium and high concentration addition levels,the recovery rates of the four compounds in livestock meat products were 87.42%～102.26%,and the relative standard deviations were 0.8%～5.93%;the recovery rates in poultry meat products were 89.76%～104.94%,the relative standard deviations were 0.62%～5.83%;the recovery rates in poultry egg products were 90.30%～106.82%,the relative standard deviations were 0.85%～7.93%;the recovery rates in aquatic products were 86.95%～107.36%,and the relative standard deviations were 0.20%～5.02%.The the method has high sensitivity and good repeatability,which providing technical reference for the detection of nitrofurans in large quantities of animal food in laboratory.
ultra high performance liquid chromatography tandem mass spectrometryanimal derived foodnitrofuran drugs
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