Study on the Changes of Polysaccharide Monosaccharide Composition from Polygonatum sibiricum in the Process of Multi-Steam-Multi-Bask
HPLC-ESLC method was used to determine the changes of polysaccharide monosaccharide composition from Polygonatum sibiricum by different batch of different steaming processes,which provided the basis for standardization evaluation of distillate extract processing technology.The results showed that fucose and fructose were detected in the polysaccharides of Polygonatum sibiricum(Polygonatum decoction pieces),processed Polygonatum sibiricum(one-steam-one-bask),processed Polygonatum sibiricum(two-steam-two-bask),processed Polygonatum sibiricum(four-steam-four-bask),processed Polygonatum sibiricum(six-steam-six-bask),and processed Polygonatum sibiricum(nine-steam-nine-bask)polysaccharides.The content of fucose in polysaccharide of nine kinds of decoction pieces was from 7.62 mg·g-1 to 3.28 mg·g-1,the content of fructose in polysaccharideand was from 5.36 mg·g-1 to 0.78 mg·g-1,the ratio of fucose to fructose of polysaccharide was from 1.42 to 4.18.The contents of fucose and fructose,as well as the ratio of fucose and fructose of polysaccharide monosaccharide in processed Polygonatum sibiricum were changed regularly with different steaming times.The above results can provide reference for the standardization of processing technology and quality evaluation of wine yellow essence.
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