Determination of Auramine O in Herbal Tea by Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry
A rapid analytical method for the determination of auramine O in herbal tea was developed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS).Acetonitrile was used as the extraction solvent,and the samples were vortex-mixed,subjected to ultrasonic extraction for 12 minutes,and then centrifuged at high speed.The supernatant was filtered through a membrane and analyzed using UPLC-MS/MS with external standard quantification.Auramine O exhibited a good linear relationship in the concentration was 0.10 to 20.00 ng·mL-1,with a correlation coefficient was 0.999 8.The method's limit of detection was 0.5 μg·kg-1,and the limit of quantification was 1.0 μg·kg-1.The recovery rate at spiking levels ranging from 2.0 μg·kg-1 to 25.0 μg·kg-1 was between 86.7%and 95.5%,with the relative standard deviation was 3.5%to 3.9%.This method demonstrates high precision,accuracy,and efficiency,making it suitable for the quantification of auramine O in herbal tea.
auramine Oultra performance liquid chromatography-tandem mass spectrometryherbal tea
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