Isolation and Identification of Lactic Acid Bacteria
In order to the valuable isolate lactic acid bacteria from natural samples of cheese and yoghurt in pastoral area,the target strains were isolated by coating and marking,and the preliminary morphological identification was carried out;the pH value,OD value and viable bacteria number of the growth process were analyzed to determine whether they were consistent with the growth characteristics of lactic acid bacteria;the strains were determined by 16S rDNA sequencing.After being compared with the NCBI database,five strains of lactic acid bacteria were obtained,they were Streptococcus thermophilus,Lactobacillus plantarum,Bifidobacterium animalis,Lactobacillus bulgaricus and Lactobacillus casei,respectively.
lactic acid bacteriaisolation and identificationflora
万方数据
维普
