食品安全导刊2024，Issue(23) ：87-90,98.

乳酸菌菌株分离鉴定

Isolation and Identification of Lactic Acid Bacteria

程凯悦 李翠平 麻士卫 孙洁宇 殷文政 闫继彪
食品安全导刊2024，Issue(23) ：87-90,98.
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  • 维普
  • 万方数据

乳酸菌菌株分离鉴定

Isolation and Identification of Lactic Acid Bacteria

程凯悦 1李翠平 1麻士卫 1孙洁宇 1殷文政 1闫继彪1
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作者信息

  • 1. 内蒙古普泽生物制品有限责任公司,内蒙古呼和浩特 010018
  • 折叠

摘要

为从牧区筛选的天然样品奶酪和酸奶中分离出有研究价值的乳酸菌,本文采用涂布、划线分离目标菌株,并对其进行初步的形态学鉴定;通过分析生长过程中的pH值、OD值、活菌数的变化规律,判定其是否符合乳酸菌生长特征;经16S rDNA测序,确定菌株类别.经NCBI数据库对比,得到5株乳酸菌,分别为嗜热链球菌(Streptococcus thermophilus)、植物乳杆菌(Lactobacillus plantarum)、动物双歧杆菌(Bifidobacterium animalis)、保加利亚乳杆菌(Lactobacillus bulgaricus)及干酪乳杆菌(Lactobacillus casei).

Abstract

In order to the valuable isolate lactic acid bacteria from natural samples of cheese and yoghurt in pastoral area,the target strains were isolated by coating and marking,and the preliminary morphological identification was carried out;the pH value,OD value and viable bacteria number of the growth process were analyzed to determine whether they were consistent with the growth characteristics of lactic acid bacteria;the strains were determined by 16S rDNA sequencing.After being compared with the NCBI database,five strains of lactic acid bacteria were obtained,they were Streptococcus thermophilus,Lactobacillus plantarum,Bifidobacterium animalis,Lactobacillus bulgaricus and Lactobacillus casei,respectively.

关键词

乳酸菌/分离鉴定/菌群

Key words

lactic acid bacteria/isolation and identification/flora

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出版年

2024
食品安全导刊
商业科技质量中心 北京肉类食品协会

食品安全导刊

影响因子：0.065
ISSN：1674-0270
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