16S rDNA鉴定腐败椰果罐头中的微生物

Identification of Microorganisms in Spoiled Coconut Cans by 16S rDNA

宋美玲 ¹郭三保 ²廖莉 ¹吴晴阳 ¹黄胜和¹

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作者信息

1. 江西中医药高等专科学校医学基础部,江西抚州 344000
2. 江西中医药高等专科学校药学系,江西抚州 344000
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摘要

以腐败椰果罐头为试材,选用 4 种培养基分离纯化腐败椰果罐头中的细菌,扩增细菌 16S rDNA,对16S rDNA序列进行同源比对,并构建系统进化树,鉴定细菌种属.结果显示,从腐败椰果罐头中分离得到38株分离菌,经16S rDNA分子生物学方法鉴定为Paenibacillus humicus、Staphylococcus warneri、Sphingomonas sp.和Liquorilactobacillus satsumensis.

Abstract

Taking spoiled coconut cans as test material,four kinds of culture media were selected to separate and purify bacteria in spoiled coconut cans,amplify bacterial 16S rDNA,compare the 16S rDNA sequences,construct a phylogenetic tree,and identify the bacterial species.The results showed that 38 isolates were obtained from spoiled coconut cans and identified by 16S rDNA molecular biology method as Paenibacillus humicus,Staphylococcus warneri,Sphingomonas sp.,Liquorilactobacillus satsumensis.

关键词

腐败椰果罐头/16S/rDNA/细菌/鉴定

Key words

spoiled coconut cans/16S rDNA/bacteria/identification

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出版年

2024

食品安全导刊

商业科技质量中心北京肉类食品协会

食品安全导刊

影响因子：0.065

ISSN：1674-0270

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