Identification of Microorganisms in Spoiled Coconut Cans by 16S rDNA
Taking spoiled coconut cans as test material,four kinds of culture media were selected to separate and purify bacteria in spoiled coconut cans,amplify bacterial 16S rDNA,compare the 16S rDNA sequences,construct a phylogenetic tree,and identify the bacterial species.The results showed that 38 isolates were obtained from spoiled coconut cans and identified by 16S rDNA molecular biology method as Paenibacillus humicus,Staphylococcus warneri,Sphingomonas sp.,Liquorilactobacillus satsumensis.
万方数据
维普
