Determination of 1,2-Propanediol and 1,3-Propanediol in Pueraria lobata Powder
Objective:To establish a method for the simultaneous detection of 1,2-propanediol and 1,3-propanediol in Pueraria lobata powder by gas chromatography-flame ionization detector(GC-FID)and gas chromatography-mass spectrometry(GC-MS).Method:The samples were extracted by acetonitrile,filtered by the extract,and then measured on the machine.Result:When GC-FID and GC-MS were used to determine the target substances,the target substances had a good linear relationship in the linear range of 0 μg·mL-1 to 50.00 μg·mL-1,0 μg·mL-1 to 10.00 μg·mL-1,respectively,and the correlation coefficients r were greater than 0.999 9;the detection limits of 1,2-propanediol were 5 mg·kg-1 and 1 mg·kg-1,the detection limits of 1,3-propanediol were 8 mg·kg-1 and 1 mg·kg-1,respectively.The average recoveries of the target substances were greater than or equal to 89.2%,and the relative standard deviations were less than 5% .Conclusion:The GC-FID method and GC-MS method have high sensitivity,high recovery,good reproducibility,simple and fast operation,and are suitable for the determination of 1,2-propanediol and 1,3-propanediol in Pueraria lobata powder,which can meet the needs of different testing institutions.
万方数据
维普
