高效液相色谱法检测软糖中褪黑素含量
Determination of Melatonin Content in Soft Candy by High Performance Liquid Chromatography
林丽炫 1张丽贤 1林岱滨1
作者信息
- 1. 广东亿超生物科技有限公司,广东汕头 515000
- 折叠
摘要
目的:建立高效液相色谱法(High Performance Liquid Chromatography,HPLC)检测软糖产品中褪黑素含量的方法.方法:软糖样品经液氮冷冻粉碎后,加 0.5 g·L-1 磷酸二氢钾溶液提取,乙腈定容,HPLC分析,外标法定量.结果:在 0.08～0.20 mg·mL-1 的浓度范围内,方法的线性良好,线性相关系数为0.999 9;方法的平均加标回收率为97.84%～100.32%,精密度为0.24%～0.41%.结论:该方法专属性良好,操作简单,结果准确,适用于含有褪黑素的软糖产品的检测.
Abstract
Objective:To establish a method for detecting melatonin in soft candy products by high performance liquid chromatography(HPLC).Method:The soft candy samples were frozen and crushed with liquid nitrogen,extracted with 0.5 g·L-1 potassium dihydrogen phosphate solution,fixed to volume with acetonitrile,analyzed by HPLC,and quantified by external standard method.Result:In the concentration range of 0.08～0.20 mg·mL-1,the method had good linearity,with a linear correlation coefficient of 0.999 9;the average spike recovery of the method was 97.84%～100.32%,and the precision was 0.24%～0.41%.Conclusion:This method has good specificity,simple operation,accurate results,and is suitable for the detection of soft candy products containing melatonin.
关键词
褪黑素/含量测定/方法研究Key words
melatonin/content determination/methods study引用本文复制引用
出版年
2024
NETL
NSTL
万方数据