Objective:A ultra performance liquid chromatography-tandem diode array method was established for the simultaneous and rapid determination of benzoic acid,sorbic acid and saccharin sodium in handcrafted milk tea.Method:The sample was dissolved in water,precipitating agent precipitated protein,extracted by ultrasound.Quasar SPP C18(100 mm×2.1 mm,2.6 μm)chromatographic column was used for isocratic elution with methanol and ammonium acetate(5+95)as mobile phase,and quantified by external standard method.Result:Three components peak within 2 minutes and the correlative coefficient of the calibration curves r＞0.999 9,the detection limit is 0.005 g·kg-1,and the quantification limit is 0.01 g·kg-1;the average recovery rates of three spiked levels of 0.01 g·kg-1、0.05 g·kg-1、0.10 g·kg-1 were 90.2%to 104.0%,and the relative standard deviation of the measurement results were 0.18%to 3.50%(n=6).Conclusion:The ultra performance liquid chromatography-tandem diode array method is simple,short detection time,sensitive and accurate,which is suitable for simultaneous and rapid determination of benzoic acid,sorbic acid and saccharin sodium in handcrafted milk tea.
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