顶空固相微萃取-气相色谱质谱法测定肉制品中的己醛
Determination of Hexanal in Meat Product by Headspace Solid-Phase Microextraction-Gas Chromatograph-Mass Spectrometry
何芸芸 1赵婷 1章荣烜 2贾健松1
作者信息
- 1. 成都市华测检测技术有限公司,四川成都 610041;四川华测检测技术有限公司,四川成都 610199
- 2. 成都市华测检测技术有限公司,四川成都 610041
- 折叠
摘要
目的:建立顶空固相微萃取结合气相色谱质谱联用法(Gas Chromatography-Mass Spectrometry,GC-MS)测定肉制品中挥发性风味物质己醛含量的方法.方法:样品在静态顶空 60℃条件下平衡 30 min达到气液平衡后,采用DVB/CAR-PDMS萃取纤维头顶空吸附30 min后进样,用DB-5ms色谱柱程序升温分离,GC-MS检测,外标法定量.结果:己醛在 100～2 000 μg·L-1 线性关系良好,相关系数为0.999 4;方法检出限为 10.0 μg·kg-1,定量限为 30.0 μg·kg-1,加标回收率为94.79%～98.06%.结论:方法的准确度高、选择性好,可用于肉制品中己醛的测定.
Abstract
Objective:To establish a method for the determination of hexanal,a volatile flavor substance in meat products,based on headspace solid-phase microextraction-gas chromatography-mass spectrometry.Method:After the sample was balanced at 60℃for 30 min to reach gas-liquid equilibrium,it was adsorbed on a DVB/CAR-PDMS extraction fiber head for 30 min before injection.The sample was separated by programmed temperature rise on a DB-5ms column,detected by gas chromatography-mass spectrometry,and quantified by external standard method.Result:Hexanal had a good linear relationship in the range of 100～2 000 μg·L-1,with a correlation coefficient of 0.999 4.The detection limit of the method was 10.0 μg·kg-1,the quantification limit was 30.0 μg·kg-1,and the spike recovery was 94.79%～98.06%.Conclusion:The method has high accuracy and good selectivity,and can be used for the determination of hexanal in meat products.
关键词
顶空固相微萃取/气相色谱质谱法/己醛/肉制品Key words
headspace solid-phase microextraction/gas chromatograph-mass spectrometry/hexanal/meat product引用本文复制引用
出版年
2024
NETL
NSTL
万方数据