Determination of Two Plant Growth Regulators in Bean Sprouts by Liquid Chromatography-Mass Spectrometry
The aim of this study was to develop a sensitive and accurate method for the determination of 6-benzyladenine and 4-chlorophenoxyacetic acid in bean sprouts by liquid chromatography-mass spectrometry with SinCHERS.The bean sprouts were extracted with acetonitrile solution containing 1%formic acid,and then the extracted salt was added to salting-out layer,which was purified by matrix dispersion purification device(SinCHERS)and separated by Agilent Zorbax Eclipse Plus C18 column,gradient elution with 0.1%formic acid 10 mmol·L-1 ammonium acetate solution and acetonitrile solution was carried out.The relationship was good(r＞0.999),the detection limit was low(about 0.14 μg·kg-1),and the recovery rate was high(85.43%to 94.51%).It provides strong technical support for monitoring and quality control of two plant growth regulators.
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