Determination of Tetracycline Antibiotics in Vegetables by QuEChERS Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
In this paper,a method for the determination of three tetracycline antibiotics in vegetables by QuEChERS combined with ultra-performance liquid chromatography tandem mass spectrometry was established.Methodological analysis showed that when the mass concentration of tetracycline was 2 ng·mL-1 to 200 ng·mL-1,the mass concentration of oxytetracycline and chlortetracycline were 1 ng·mL-1 to 200 ng·mL-1,the linear relationship was good,and the linear coefficients were greater than 0.995.The limits of detection of tetracycline,oxytetracycline and aureomycin were 0.8 μg·kg-1,0.4 μg·kg-1 and 0.4 μg·kg-1,and the limits of quantitation were 2.6 μg·kg-1,1.3 μg·kg-1 and 1.3 μg·kg-1,respectively.The recoveries of the target compounds were 64.5%to 92.6%and the relative standard deviations were 1.8%to 10.9%The results indicated this method is sensitive and easy to operate,which is suitable for the rapid determination of there tetracycline antibiotics in vegetables.
vegetablesultra performance liquid chromatography-tandem mass spectrometrytetracycline antibiotics
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