Determination of Nitrofuran Metabolites in Sea Cucumber by Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry
Objective:To establish a rapid,accurate,and efficient method for detecting residual nitrofuran metabolites in sea cucumber using ultra-high performance liquid chromatography tandem mass spectrometry.Method:Sample pretreatment was carried out using ultrasound for 1 hour in a 40℃water bath,derivatization for 5 hours in a 45℃gas bath,and pH values ranging from 7.0 to 7.5.Gradient elution was performed using a mobile phase of 2 mmol·L-1 ammonium acetate in 0.1%formic acid aqueous solution acetonitrile,and ultra-high performance liquid chromatography tandem mass spectrometry was used for determination.Result:The four nitrofuran metabolites showed a good linear relationship in the range of 0.5 ng·mL-1 to 20.0 ng·mL-1,with an average recovery rate of 97.3%to 103.6%.The detection limit of the method was 0.5 μg·kg-1,and the quantification limit was 1.0 μg·kg-1.Conclusion:This method can be used for rapid detection of residual nitrofuran metabolites in commercially available sea cucumbers.
sea cucumbernitrofuran metabolitesultra high performance liquid chromatography-tandem mass spectrometry
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