婴幼儿食品和乳粉中糖类含量的测定
Determination of Sugars in Food and Milk Powder for Infants and Young Children
李小娟1
作者信息
- 1. 甘肃省陇西县妇幼保健院 儿科,甘肃陇西 748100
- 折叠
摘要
目的:建立高效液相色谱测定婴幼儿食品和乳粉中糖类含量的方法.方法:采用氨基色谱柱(250 mm×2.1 mm,5 μm)进行分离,以乙腈∶水(85∶15,V/V)为流动相,等度洗脱,外标法定量.结果:果糖、葡萄糖、蔗糖及乳糖含量在 0.5～15.0 mg·mL-1 线性关系良好,回收率为 77.1%～104.3%,相对标准偏差为 1.2%～6.0%(n=6).结论:本方法提取效率高、操作简单、结果准确,适用于婴幼儿食品和乳粉中果糖、葡萄糖、蔗糖及乳糖含量的测定.
Abstract
Objective:To establish a method for the determination of sugars in food and milk powder for infants and young children by high performance liquid chromatography.Method:The separation was carried out on an amino-column(250 mm×2.1 mm,5 μm)with acetonitrile∶water(85∶15,V/V)as the mobile phase,and quantitative analysis by external standard method.Result:The linear relationship of fructose,glucose,sucrose and lactose contents was good in the range of 0.5 mg·mL-1 to 15.0 mg·mL-1,the average recoveries were 77.1%to 104.3%,the relative standard deviations were 1.2%to 6.0%(n=6).Conclusion:This method has high extraction efficiency,simple operation and accurate results,and is suitable for the determination of fructose,glucose,sucrose and lactose in infant food and milk powder.
关键词
糖类/高效液相色谱法/婴幼儿食品和乳粉Key words
sugars/high performance liquid chromatography/infants and young children引用本文复制引用
出版年
2024
NETL
NSTL
万方数据