Determination of Sugars in Food and Milk Powder for Infants and Young Children
Objective:To establish a method for the determination of sugars in food and milk powder for infants and young children by high performance liquid chromatography.Method:The separation was carried out on an amino-column(250 mm×2.1 mm,5 μm)with acetonitrile∶water(85∶15,V/V)as the mobile phase,and quantitative analysis by external standard method.Result:The linear relationship of fructose,glucose,sucrose and lactose contents was good in the range of 0.5 mg·mL-1 to 15.0 mg·mL-1,the average recoveries were 77.1%to 104.3%,the relative standard deviations were 1.2%to 6.0%(n=6).Conclusion:This method has high extraction efficiency,simple operation and accurate results,and is suitable for the determination of fructose,glucose,sucrose and lactose in infant food and milk powder.
sugarshigh performance liquid chromatographyinfants and young children
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