Determination of Mirodenafil and Gendenafil in the Heathy Food by UPLC-MS
To establish a method for determination of mirodenafil and gendenafil illegally added in the relieve fatigue heathy food by UPLC-MS.Mirodenafil quantitive product ion 99.1 and qualitative product ion 296.1,gendenafil quantitive product ion 285.2 and qualitative product ion 327.1 were detected as target ions under the positive mode multiple reaction monitoring,and quantitative analysis used blank matrix external standard method.The results indicated that the blank matrix solution had no interference.Milonafil and gendenafil had a good linear relationship within the range of 10～1 000 ng·mL-1,with a correlation coefficient of 0.999 7 and 0.999 3,respectively.The precision of the method for mironafine is 1.5%,average recovery rates were from 90.4%to 97.9%,and the relative standard deviation were between 0.9%to 1.8%.The precision of the method for gendenafil is 1.2%,average recovery rates were between 89.4%to 96.6%,with relative standard deviation of 0.6%to 2.4%.The method detection limit and quantification limit are 50 µg·kg-1 and 100 µg·kg-1,respectively.The method is high specificity,accurate and sensitive,can be used as a trace analysis method for illegally adding mirodenafil and gendenafil in fatigue relieving health foods.
mirodenafilgendenafilultra high performance liquid chromatography-mass spectrometry(UPLC-MS)heathy foodillegally added
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NSTL
万方数据
