HPLC-MS法同时检测鱼肉中32种兽药残留
Simultaneous Determination of 32 Veterinary Drugs in Fish by High performance Liquid Chromatography-Tandem Mass Spectrometry
王延龙 1黄雷鹏 1韩学功 1王兆全1
作者信息
- 1. 昌邑市检验检测中心,山东昌邑 261300
- 折叠
摘要
用PLS-A固相萃取小柱处理鱼肉样品,采用高效液相色谱-串联质谱法对鱼肉中的 32 种兽药残留进行测定.实验优化了样品提取方法、色谱条件,并研究了方法的线性范围、准确度、精密度以及基质效应.结果表明,32 种兽药的回收率在 75.3%～114.7%,检出限为 1.0 μg·kg-1,相对标准偏差均小于 15%(n=6),32 种兽残在 1～100 ng·mL-1 线性关系良好,相关系数均高于 0.991.该方法准确度高、精密度好,可为鱼肉中 32种兽药残留的检测提供技术支撑.
Abstract
In this experiment,a PLS-A solid-phase extraction column was used to treat fish meat samples,and high-performance liquid chromatography tandem mass spectrometry was used to determine the residues of 32 veterinary drugs in fish meat.The experiment optimized the sample extraction method and chromatographic conditions,and studied the linear range,accuracy,precision,and matrix effects of the method.The results showed that the recovery rates of 32 veterinary drugs ranged from 75.3%to 114.7%,with a detection limit of 1.0 μ g·kg-1 and a relative standard deviation of less than 15%(n=6),there was a good linear relationship between the 32 animal residues in the range of 1～100 ng·mL-1,with correlation coefficients higher than 0.991.This method has high accuracy and good precision,and can provide technical support for the detection of 32 veterinary drug residues in fish meat.
关键词
高效液相色谱-串联质谱法(HPLC-MS)/兽药残留/PLS-A/鱼肉Key words
high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS)/veterinary drug residues/PLS-A/fish引用本文复制引用
出版年
2024
NETL
NSTL
万方数据