Simultaneous Determination of 32 Veterinary Drugs in Fish by High performance Liquid Chromatography-Tandem Mass Spectrometry
In this experiment,a PLS-A solid-phase extraction column was used to treat fish meat samples,and high-performance liquid chromatography tandem mass spectrometry was used to determine the residues of 32 veterinary drugs in fish meat.The experiment optimized the sample extraction method and chromatographic conditions,and studied the linear range,accuracy,precision,and matrix effects of the method.The results showed that the recovery rates of 32 veterinary drugs ranged from 75.3%to 114.7%,with a detection limit of 1.0 μ g·kg-1 and a relative standard deviation of less than 15%(n=6),there was a good linear relationship between the 32 animal residues in the range of 1～100 ng·mL-1,with correlation coefficients higher than 0.991.This method has high accuracy and good precision,and can provide technical support for the detection of 32 veterinary drug residues in fish meat.
high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS)veterinary drug residuesPLS-Afish
NETL
NSTL
万方数据
