Simultaneous Determination of 4 Kinds of Oxyphenisatin in Food by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
An analytical method for the simultaneous determination of four oxyphenisatin in food by ultra-performance liquid chromatography-tandem mass spectrometry was established.Samples were extracted with 80%methanol,separated on a Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8 μm)column,eluted with acetonitrile-0.1%formic acid solution,and analyzed by a triple quadrupole mass spectrometer.The results showed that the four oxyphenisatin concentrations were 1.0 ng·mL-1 to 100.0 ng·mL-1 with good linear relationship,the correlation coefficients were greater than 0.999;the detection limit is 10 μg·kg-1,and the quantification limit is 20 μg·kg-1,the recoveries were 85.7%to 94.5%,and the relative standard deviations were 2.9%to 5.0%.This method has high sensitivity and accuracy,and is suitable for the detection of four oxyphenisatin in food.The method was applied to 30 different types of food on the market,and a total of 3 positive samples were screened,with a detection rate of 10.0%.
food safetyillegal additivesoxyphenisatinultra-performance liquid chromatography-tandem mass spectrometry
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