Establishment of a Loop-Mediated Isothermal Amplification for Visualization of Enteroinvasive Escherichia coli
Enteroinvasive Escherichia coli(EIEC)is an important foodborne pathogen that causes intestinal diseases in humans.In this study,we established a visual loop-mediated isothermal amplification(LAMP)method,designed primers for the EIEC specific gene ipaH,optimized the reaction conditions,and evaluated the specificity and sensitivity of the method.The results showed that the established LAMP method could be used to determine the results after 45 min of reaction at 63℃ by naked eye or under ultraviolet light,and the sensitivity of the method for the detection of EIEC was up to 10 pg·μL-1.The method had good specificity,and there was no cross-reactivity with other common intestinal pathogens,such as Salmonella,Vibrio parahaemolyticus,and Staphylococcus aureus,etc.The method also showed no cross-reactivity with other common intestinal pathogens,such as Vibrio parahaemolyticus.The visualized LAMP method established in this study is rapid,sensitive,and requires low equipment,which can meet the needs of rapid detection in the field.
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